Journal: PeerJ

Article Title: Platelet factor 4 inhibits human hair follicle growth and promotes androgen receptor expression in human dermal papilla cells

doi: 10.7717/peerj.9867

Figure Lengend Snippet: (A–H) Effects of PF4 on hair growth-promoting and DP signature genes ( Wnt5a, Wnt10b , DKK1 , LEF1, HEY1, IGF-1, BMP2 and BMP4 ) mRNA expression in human DPCs cultured for 24 h. (I–K) Effects of PF4 on protein expression of Wnt5a, IGF-1 and BMP2 in human DPCs cultured for 48 h. Data are reported as mean + SEM. Student’s t-test was used to compare data. * P < 0.05, ** P < 0.01. “ns” indicates no significant difference.

Article Snippet: Wnt5a, IGF1 and BMP2 were measured by Wnt5a, IGF1 and BMP2 ELISA kits (CUSABIO) following the manufacturer’s instruction, respectively.

Techniques: Expressing, Cell Culture

Journal: Journal of Translational Medicine

Article Title: UBC9-mediated regulation of K144 ubiquitination of Lamin A and its implications for hepatocellular carcinoma

doi: 10.1186/s12967-026-07722-0

Figure Lengend Snippet: Impact of UBC9 knockdown on HCC cells using a doxycycline-inducible system. ( A ) schematic of the doxycycline (Dox)-inducible gene repression system used to downregulate UBC9 expression. ( B ) Western blot analysis shows reduced UBC9 protein levels after 72-hour of Dox treatment. ( C ) colorimetric CCK8 proliferation assay reveals significantly decreased cell proliferation in UBC9-depleted cells over 72 hours. ( D ) scratch assay shows reduced cell migration in UBC9 knockdown cells, 48 hours post-Dox induction. ( E ) Matrigel invasion assay demonstrates a marked decrease in the invasive capacity of HCC cells upon UBC9 knockdown. ( F ) Western blot analysis and quantification confirming UBC9 knockdown efficiency in Huh-7 cells. ( G ) CCK-8 assay revealing the effect of UBC9 depletion on Huh-7 cell proliferation. ( H ) wound healing assay showing the impact of UBC9 knockdown on the migration of Huh-7 cells. ( I ) Transwell invasion assay demonstrating the effect of UBC9 knockdown on the invasion of Huh-7 cells. ( J ) in vivo xenograft tumor assay. Images of dissected tumors, tumor weights, and tumor volume growth curves from nude mice injected with control (NC) or UBC9-shRNA Hep3B cells. ns, p > 0.05; **, p < 0.01; ***, p < 0.001

Article Snippet: UBC9 shRNA and scrambled control shRNA sequences were cloned into the pTRIPZ lentiviral vector containing a tetracycline (doxycycline, Dox)-inducible system (Thermo Scientific, Waltham, MA, USA).

Techniques: Knockdown, Expressing, Western Blot, Proliferation Assay, Wound Healing Assay, Migration, Invasion Assay, CCK-8 Assay, Transwell Invasion Assay, In Vivo, Injection, Control, shRNA